URINALYSIS AND URINE MICROSCOPY

Aim: To detect various components in a urine sample.
 
Principle: It’s a quantitative test used to checkout for parameters like blood, Ketone, glucose, protein, nitrate, bilirubin, PH, Urobilinogen, and ascorbic acid in urine sample. 


Materials

Urine sample

Combi 9 strip

Test tube

Bucket centrifuge

Microscope

Cover slip

Glass slide

Colour chat


Procedure

A universal bottle is given to the patient to urinate into it, 2/3 full, place a strip of Combi 9, into the fresh urine sample, remove the stripe and hit it on the edge of the container to remove urine, then compare the color of the Combi 9 strip with that on the color chat.



Result:

 The parameters to be checked out for are glucose, blood, Ketone, protein, nitrate, bilirubin, PH and Urobilinogen, are all on the Combi 9strip.

The result is base on color change of these parameters on the strip.


Urine can also be examined microscopically to check for color and appearance (cloudy/clear).


Microscopy

Aim: To detect the presence of infection in the urinary tract.


Procedure

Fill 1/3 test tube with urine

Centrifuge the urine for sedimentation to take place

Decant the supernatant

Place the sediments into a grease free slide

Mount the slide of the stage, secure it with a stage clip and view using x10 objective and then switch to x100 for more details.


Result

White blood cell, pus cells, epithelia cells and yeast cells can be seen. Also parasites such as Taenia Vaginalis (TV), Schitosoma Haematobium and others can be detected.

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